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. 2022 Jun 24;3(3):101507. doi: 10.1016/j.xpro.2022.101507

Figure 6.

Figure 6

Troubleshooting

(A) Images of different stages during culture of OTCs with an appropriate distance – DIV 0, 1, 7, 14, 21. DIV: day in vitro (where the day of the dissection is DIV0).

(B) OTCs placed too close together will touch when flattening. DIV: day in vitro (where the day of the dissection is DIV0). Scale bar: 2 mm.

(C) Illustration shows correct (left) and incorrect (right) orientation of the gel during embedding for imaging in an upright microscope. Misalignment of the gel and positioning the sample far from the objective will result in significant distortion due to the gel.