∗Monomer solution

Reagent	Final concentration	Amount
Sodium acrylate	86 g/l	2.25 mL
Acrylamide	25 g/l	0.86 mL
N,N-methylenebisacrylamide	1.5 g/l	0.75 mL
NaCl (5 M)	117 g/l	4 mL
10× DPBS	1×	1 mL
Water	n/a	0.54 mL
Total		9.4 mL

Divide in 1 mL aliquots and store at −20°C. Do not freeze after thawing an aliquot; a thawed aliquot can be used up to several weeks later.