REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-GluA2 (extracellular epitope, clone 6C4, 1:500)	Millipore	Cat # MAB397; RRID: AB_2113875
Chicken polyclonal anti-GFP (1:1000)	Abcam	Cat # ab13970; RRID: AB_300798
Donkey polyclonal anti-chicken Alexa 488 (1:500)	Dianova	Cat# 703-546-155; RRID: AB_2340376
Donkey polyclonal anti-mouse Alexa 568 (1:500)	Molecular Probes	Cat # A10037; RRID: AB_2534013
Chemicals, peptides, and recombinant proteins
4-hydroxy-2,2,6,6-tetramethylpiperidin-1-oxyl (4-Hydroxy-TEMPO)	Sigma	Cat# 176141; CAS 2226-96-2
4-(2-hydroxyethyl)-1- piperazineethanesulfonic acid (HEPES)	Invitrogen	15630-056
45% D-(+)-Glucose	Sigma	Cat# G7869; CAS 50-99-7
Acrylamide	AppliChem	Cat# A0951; CAS 79-06-1
Acryloyl-X SE	Thermo Fisher Scientific	Cat# A20770; CAS 63392-86-9
Agarose, low-melt	Roth	Cat# 6351.2: CAS 39346-81-1
Ammonium persulfate (APS)	Bio-Rad	Cat# 161-0700; CAS 7727-54-0
B-27 supplement	Invitrogen	17504-044
Basal Medium Eagle (BME)	Invitrogen	41010-026
Dimethylsulfoxide (DMSO), anhydrous	Sigma	276855
Dulbecco’s phosphate-buffered saline (DPBS)	Invitrogen	14190-169
Donor Horse serum	Sigma	Cat# 1138
Ethylenediaminetetraacetic acid (EDTA)	Calbiochem	Cat# 1032456; CAS 6381-92-6
GlutaMax	Gibco	35050-038
Modified Eagle Medium (MEM)	Invitrogen	21575-022
N-2 supplement	Invitrogen	17502-048
N,N-methylenebisacrylamide	Sigma	Cat# 146072; CAS 110-26-9
Penicillin/Streptomycin	Gibco	15140-122
Proteinase K	Roche	Cat# 03115828001; EC 3.4.23.1
Sodium acrylate	Sigma	Cat# 408220; CAS 7446-81-3
Sodium bicarbonate	Roth	HN01.1
Sodium chloride	Roth	HN00.2
Tetramethylethylenediamine (TEMED)	Roth	Cat# 2367.3; CAS 110-18-9
Tris-HCl	Roth	9090.3
Triton X-100	Merck	Cat# 1.2298.0101; CAS 9002-93-1
Experimental models: Organisms/strains
Mouse: Tg(Thy1-EGFP)MJrs (male/female, 3–6 days old)	Feng et al. (2000)	MGI ID: 3766828
Software and algorithms
Huygens Professional 17.04–19.10	Scientific Volume Imaging	https://svi.nl/HomePage
ImageJ 5.12	Schneider et al. (2012)	https://imagej.nih.gov/ij/
Imaris 9.0–9.6	Bitplane	https://imaris.oxinst.com/
Other
35 mm dishes, uncoated, coverslip No 1, 14 mm glass diameter, with microwell	MatTek	Cat# P35G-1.0-14-C
Acrylate glue	Ellsworth Adhesives	Permabond 102
Coverslips (12/13 mm diameter)	Marienfeld	Cat# 01-115-30
Dumont #7 - Fine Forceps	Fine Science Tools	Cat# 11274-20
Mayo Scissors - ToughCut®	Fine Science Tools	Cat# 14110-17
Millicell cell culture inserts	Merck	Cat# PICMORG50
Sichel knife	Fine Science Tools	Cat# 10073-14
Spatula	Hammacher	Cat# HWN034-15
Spring Scissors - 8 mm Cutting Edge	Fine Science Tools	Cat# 15024-10
Student Dumont #5 Forceps	Fine Science Tools	Cat# 91150-20
Two-channel laser-scanning confocal microscope	Leica	TCS SP8
Vibratome	Leica	VT1200S