FIGURE 4.

Exosomes were successfully isolated and identified out from LUAD cell culture media, and the exosomal miR‐16‐5p was lower expressed in the LUAD cell culture media. (A) The morphology of exosomes in cell culture media of A549, PC9, HCC827, and BEAS‐2B cell under TEM. Scale bars: 200 nm. (B) The protein level of exosomal surface marker protein of TSG101 and CD63 in exosomes in the cell culture media of A549, PC9, HCC827, and BEAS‐2B cell by WB. (C) The miR‐16‐5p expression in exosomes in the cell culture media of A549, PC9, HCC827, and BEAS‐2B cell with qRT‐PCR. *p < 0.05; ** vs. BEAS‐2B, p < 0.01. exo, exosomes; LUAD, lung adenocarcinoma