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. 2022 Mar 28;11(13):2627–2643. doi: 10.1002/cam4.4638

FIGURE 5.

FIGURE 5

Upregulation of exosomal miR‐16‐5p in the cell culture medium restrained cell proliferation and migration, and accelerated cell apoptosis. (A) The relative miR‐16‐5p expression in HCC827 cells transfected into NC mimic or miR‐16‐5p mimic with qRT‐PCR. (B) The exosomal miR‐16‐5p level in the cell culture medium from HCC827 cells transfected into NC mimic or miR‐16‐5p mimic with qRT‐PCR. (C) Cell proliferation was estimated by BrdU immunostaining after treatment with exosomes from the HCC827/PC9 cell culture media transfected with NC mimic/miR‐16‐5p mimic/miR‐16‐5p mimic+PD‐L1 inhibitor. Red, HCC827/PC9 cells labeled with BrdU; blue, nuclei counterstained by BrdU. Scale bar, 50 μm. (D) The assessment of cell proliferation via colony formation assays after treatment. (E) Cell migration was determined with the wound healing assay at 0 and 24 h after treatment. Scale bar, 200 μm. (F) Cell apoptosis was assessed by flow cytometry after treatment. Q1‐UR: upper‐right side, early cell apoptosis. Q1‐LR: lower‐right side, late cell apoptosis. ** vs. NC mimic, p < 0.01; ** vs. NC mimic‐HCC827/PC9‐exosomes, p < 0.01; *** vs. NC mimic‐HCC827/PC9‐exosomes, p < 0.001; ## vs. miR‐16‐5p mimic‐ HCC827/PC9‐exosomes, p < 0.01; ### vs. miR‐16‐5p mimic‐HCC827/PC9‐exosomes, p < 0.001. NC, negative control; BrdU, 5‐bromo‐2′‐dexoyuridine; PD‐L1, programmed cell death ligand‐1