Figure 3 |. InterOrgan communication via vascularized barrier of tissue-specific niches.

a-c, Characterization of the vascular barrier insert (a) at various pore size (b) as measured by vascular transendothelial electrical resistance (TEER) values (c). (n=7–8 biological replicates) d, TEER measurements as a function of shear stress (n=4 biological replicates). Data is shown as mean ± SD and statistics determined by one-way ANOVA. e, Cardiac tissues contain a majority of GFP⁺CD63⁺ cardiomyocytes, as indicated by expression of cardiac troponin (cTnT) and CD63 in quadrant 3 of the flow cytometry scatter plot. f-g, Average expression (f) (n=5 biological replicates) and tissue heat-map expression (g) of GFP⁺CD63 within all tissues cultured in the InterOrgan tissue chip for 2 weeks. h, Immunofluorescence image of GFP⁺CD63 expression within the vascular barrier beneath the cardiac tissue after 2 weeks. i-j, Average expression (i) and tissue heat-map expression (j) of labelled monocytes within all tissues cultured in the InterOrgan or Mixed tissue chips for 24 hours (n=3 biological replicates). k, Immunofluorescence image of CD14⁺ monocytes attached to the vascular barrier beneath the cardiac tissue after 24 hours. Scale bar, 10 μm. l, Cardiac troponin concentration within each tissue chamber 24 hours after cardiac cryoinjury (n=3 biological replicates). m, Monocyte infiltration over time as measured by confocal tracking of labelled monocytes (n=3 biological replicates). n, Immunofluorescence image of CD14⁺ monocytes (green) attached to a cryoinjured cardiac tissue counterstained with DAPI (blue) after 7 days. Scale bar, 10 μm o-p, Tissue heat-map expression (o) and average expression (p) of labelled monocytes within healthy and cryoinjured cardiac tissues after 7 days (n=4 biological replicates). Data is shown as mean ± SD and statistics determined by unpaired t-test.