REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-Myosin Heavy Chain antibody, clone A4.1025	Millipore	Cat#05–716; RRID:AB_309930
Anti-Tead1 (TEF-1 Pure)	BD Biosciences	Cat# 610922; RRID:AB_398237
Anti-IgG	Millipore	Cat# Magna 0014
Anti-APJ receptor antibody	Abcam	Cat# ab214369
Anti-Apelin antibody	Abcam	Cat# ab125213 RRID:AB_10999708
Anti-Laminin antibody	LS Bio	Cat# LS-C96142 RRID:AB_2033342
Anti-CD31 (Pecam1) antibody	BD Biosciences	Cat# 557355 RRID:AB_396660
Critical commercial assays
One-Glo Firefly Luciferase Detection Kit	Promega	Cat# E8120
Nano-Glo Dual-Luciferase Detection Kit	Promega	Cat# N1620
Glo-lysis Buffer	Promega	Cat# E2661
Lipofectamine RNAiMax	Thermofisher	Cat# S-006-100
Apelin EIA kit	Phoenix pharmaceuticals	Cat# EK-057-23
Gibson assembly kit	NEB	Cat# E5510
FastLane cell multiplex NR kit	Qiagen	Cat# 216713
miRNeasy Mini Kit	Qiagen	Cat# 217004
Covaris truChIP chromatin shearing kit	Covaris	Cat# SKU:500465
Magna ChIP A/G kit	Millipore/Sigma	Cat#17–10085
Minielute DNA purification kit	Qiagen	Cat# 28004
Maxima Sybr Green/ROX master mix	Thermo Scientific	Cat# K0221
Chemicals, peptides, and recombinant proteins
Recombinant Phy Apelin-13	Bachem	Cat# U-01260
Cardiotoxin	Laxotan	Cat# L8102
Notexin	Laxotan	Cat# L8104
Deposited data
GSE1133	bioGPS	GeneAtlas UI33A (gcrma) for human samples
GSE1133	bioGPS	GeneAtlas MOE430 (gcrma) for mouse tissues
GSE45577	GEO	(Lukjanenko et al., 2013) https://doi.org/10.1371/journal.pone.0071084
GSE143435, GSE143437, GSE159500	GEO	(McKellar et al., 2021) https://doi.org/10.1101/2020.12.01.407460
Experimental models: Cell lines
C2C12 myoblast cell line	ATCC	Cat# CRL1772 RRID:CVCL_0188
C166 endothelial cell line	ATCC	Cat# CRL2581 RRID:CVCL_6581
EOMA endothelial cell line	ATCC	Cat# CRL2586 RRID:CVCL_3507
C166GFP endothelial cell line	ATCC	Cat# CRL2583 RRID:CVCL_6582
Experimental models: Organisms/strains
C57BL/6J – 24month old	Jackson	Cat# 000664 RRID:IMSR_JAX:000664
Mck-Tead1 OE mice		(Southard et al., 2016)
Oligonucleotides
si-RNA scramble pool	Dharmacon	Cat# D-001810-10–50
si-Tead1 pool	Dharmacon	Cat# L-048419-01–0005
si-Zdhhc9 pool	Dharmacon	Cat# L-058018-01–0005
si-Zfp319 pool	Dharmacon	Cat# L-059731-01–0005
Apln taqman probe	Life Technologies	Mm00443562_m1
Hprt taqman probe	Life Technologies	Mm00446968_m1
Tead1 taqman probe	Life Technologies	Mm00493507_m1
Barx1 taqman probe	Life Technologies	Mm01353100_m1
Zic3 taqman probe	Life Technologies	Mm00494362_m1
Zdhhc9 taqman probe	Life Technologies	Mm00552609_m1
Gcm2 taqman probe	Life Technologies	Mm00492312_m1
Pecam1 taqman probe	Life Technologies	Mm01242576_m1
Icam 1 taqman probe	Life Technologies	Mm00516023_m1
Tek (Tie2) taqman probe	Life Technologies	Mm00443243_m1
Recombinant DNA
N/A
Software and algorithms
SnapGene	Snapgene	N/A
metaXpress	Molecular Devices	N/A
VS-ASW FL software	Nikon	N/A
MetaXpress software	Molecular Devices	N/A
NIS-Elements	Nikon	N/A
GraphPad Prism Software	GraphPad	N/A
ApE	ApE	version 7–9
Transcription factor-DNA interaction detection in yeast (TIDY)	(Hens et al., 2011)	https://updeplasrv1.epfl.ch/software/ https://doi.org/10.1038/nmeth.1763
Other
Seurat	(Butler et al., 2018)	version3.1.5
Harmony	(Korsunsky et al., 2019)	version 1.0