Fig. 3.

Localization of the cell polarity regulator APC in migrating T cells. Immunofluorescence of a primary human CD8 T cell migrating on a VCAM-1+chemokine-adhesive substrate, with a distinguishable polarized shape. Contrary to other polarity regulators, APC (puncta in cyan) does not preferentially localizes at a pole of the cells, but rather distributes all along the entire microtubule network (filaments in red), from the centrosome to both the leading edge and the uropod. Inset shows a zoom of the framed region. Arrow shows the direction of migration.