Extended Data Figure 3. Recognition of cancer cell antigens by NK1.1⁺ αβILTCk-TCRs.

a, A representative flow plot showing the gating strategy used to isolate NK1.1⁺ αβILTCks and PD-1⁺ T cells (TCs) from PyMT mice. NK1.1⁺ αβILTCks are identified as CD45⁺TCRβ⁺CD8α⁺PD-1⁻NK1.1⁺ whereas PD-1⁺ TCs are defined as CD45⁺TCRβ⁺CD8α⁺PD-1⁺NK1.1⁻. b, A histogram showing the distribution of CDR3 lengths of TCRα and TCRβ pairs isolated from αβILTCks and PD-1⁺ TCs. Data are pooled from two independent experiments. c, Flow cytometric analysis showing the frequency of GFP⁺ cells among CD8⁺ and CD8⁻ reporter cell line expressing indicated TCRs 24 hours after co-culturing with primary PyMT cancer cells or splenocytes pulsed with the SIINFEKL peptide (OVA_257–264). Data are representative of three independent experiments. d, Frequency of GFP⁺ cells among CD8⁻ reporter cell line expressing indicated NK1.1⁺ αβILTCk-derived TCRs 24 hours after co-culturing with primary PyMT cancer cells. n = 3 for each TCR. All statistical data are shown as mean ± S.D (biologically independent mice in a-c and independent samples in d).