Effect of nitrite on expression of proinsulin (A) and C-peptide (B) in the pancreatic tissues of Type 2 diabetic rats. Representative images of pancreatic tissue immune fluorescence stained with DAPI (blue, DNA stain), proinsulin, and C-peptide (green) as well as quantification of immune fluorescence staining by imageJ analysis are shown. Scale bar = 100 mm. Values are mean ± SEM (n = 6/group). ∗, † significant difference (p values < 0.05) compared with control and diabetes groups, respectively.