FIGURE 1.

Effect of early life growth hormone (GH) intervention on level of BDNF and Doublecortin (DCX) in hippocampus of Ames dwarf mice. (A,C) Cell lysate was prepared from hippocampus of 15–18 months old wild‐type (WT) littermate control male mice and long‐lived Ames dwarf male mice (DF) with or without GH treatment. Protein levels of BDNF (Panel A) or DCX (Panel C) were then measured by western blotting. Representative gel images are shown. (B,D) Protein quantification data normalized to β‐actin and expressed as fold change compared with WT control (defined as 1.0). N = 8 or 9 mice for each group. *p < .05 and **p < .01 by Tukey's post‐hoc test after one‐way anova.