FIGURE 2.

Effect of early life growth hormone intervention on level of uncoupling protein 1 (UCP1) in adipose tissue of Ames dwarf mice. (A) Cell lysate was prepared from brown adipose tissue (Panel A), inguinal fat (Panel C), or perigonadal fat (Panel E) of wild‐type (WT) and DF mice 15–18 months old and evaluated for UCP1 protein by western blotting. Representative gel images are shown. (Panels B,D,F) Protein quantification data normalized to β‐actin and expressed as fold change compared with WT control (defined as 1.0). N = 8 or 9 mice for each group. *p < .05 and **p < .01 by Tukey's post‐hoc test after one‐way anova.