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. Author manuscript; available in PMC: 2023 Jan 1.
Published in final edited form as: Methods Mol Biol. 2022;2394:601–616. doi: 10.1007/978-1-0716-1811-0_32

Fig. 5.

Fig. 5.

Time course of eGFP expression in HEK293 cells after transduction with mesh disks formulated with AAV2-eGFP immobilized according to the PABT/PEI(PDT)/protein G/anti-AAV2 antibody tethering protocol. Serial fluorescence microscopy (FITC filter set; 40 × original magnification) of HEK293 cells transduced with AAV2-eGFP meshes (A, upper panel) versus control meshes (A, lower panel) at the time of mesh disk placement, and 1, 3 and 7 days post-transduction supplemented by fluorimetry of the respective wells (B).