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. 2022 Jun 28;15:3381–3393. doi: 10.2147/IDR.S364388

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© 2022 Chen et al.

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The percentage of CD4⁺ T cells secreting IFN-γ in PBMCs of 5 patients at Day 10 following in vitro stimulation with heat-killed T. marneffei. (A) presents flow cytometry plots (TM2, TM5, TM11, TM12, and TM13 represent PBMCs from 5 patients; P1-P3 represent serum from 3 anti-IFN-γ autoAb-positive patients (TM1, TM2, TM3); HC represents serum from 1 healthy control; NC represents the negative control. (B) shows the significant difference of the percentage of IFN-γ⁺ CD4⁺ T cells between the anti-IFN-γ autoAb-positive serum and normal serum groups. (C) and (D) shows no significant difference of the percentage of IFN-γ⁺ CD4⁺ T cells between anti-IFN-γ autoAb-positive and negative patients with either high titer IFN-γ autoantibody serum or HC serum culture.