FIGURE 11:
Glucose starvation and Gpa2 overexpression. (A, B) Wild-type cells expressing GFP-Snx41 (A) and GFP-Pib1 (B) were imaged in glucose-replete media, following 2 h raffinose exchange, and in cells overexpressing Gpa2. Quantifications for each experiment were performed (right) by comparing the distribution of GFP-Snx41 and GFP-Pib1 dots in each cell as a percentage. Unpaired Student’s t tests were performed, with asterisk (*) indicating p < 0.001. Scale bar, 5 µm.
