TABLE 1:
One set of parameters for each of the three models.
| Associations (l/mol s) | Dissociations (/s) | Transformations (/s) | Initial conditions | |
|---|---|---|---|---|
| Figure 4C. RasGAP activated by Ga2 and RasGTPa | Ligand_GPCR binding—10,000,000 | Ligand_act-GPCR dissoc—1 | GPCR-basal: 50 | |
| Ligand_GPCR Receptor_Gabg | Receptor_Gabg binding—50,000 | rec act ga gdp dissoc—0.01 | GPCR activation—3 | Ga2_Gbg_3 basal: 50 |
| rec act ga gtp dissoc—10 | Ras-GDP: 50 | |||
| rec inact ga dissoc—10 | RasGAP_basal: 1e-06 | |||
| Ga2_Gbg | GDP-Ga_Gbg assoc—1e-06 | Ga-GTP_Gbg dissoc—10 | ||
| gabg-gdp dissoc—1e-03 | ||||
| Ga2 | G alpha auto GTPase—3 | |||
| Ga2_Rasgap | Ga2-GDP_RasGAP inact assoc—0.01 | Ga2-GTP_RasGAP dissoc—2e-03 | Ga2-GTP_RasGAP activation—100 | |
| Ga2-GTP_RasGAP assoc—0.01 | Ga2-GDP_RasGAP inact dissoc—2e-03 | |||
| RasGAP_Ga2-GTP assoc—1e06 | ||||
| Gbg_Ras | Gbg_Ras assoc—1e-06 | Gbg_Ras dissoc—1e-03 | Gbg_Ras activation—100 | |
| Ras_RasGAP | Ras_RasGAP assoc—1e-08 | Ras-GTP_RasGAP dissoc—1e-03 | Ras-GTP_RasGAP activation—100 | |
| RasGAP_RasGTP assoc—1e-05 | Ras_RasGAP dissoc—1e-03 | Ras_RasGAP deactivation—1e-03 | ||
| RasGDP_RasGAP inactive assoc—-0.1 | RasGDP_RasGAP inactive dissoc—0.1 | |||
| RasGAP | RasGAP deactivation—0.03 | |||
| Figure 4A. RasGAP is activated by Ga2b | ||||
| Ligand_GPCR | Ligand_GPCR binding— 10,000,000 | Ligand_act-GPCR dissoc—1 | GPCR-basal: 50 | |
| Receptor_Gabg | Receptor_Gabg binding—50,000 | rec act ga gdp dissoc—0.01 | GPCR activation—3 | Ga2_Gbg_3 basal: 50 |
| rec act ga gtp dissoc—10 | Ras-GDP: 50 | |||
| rec inact ga dissoc—10 | RasGAP_8: 0.0001 | |||
| Ga2_Gbg | GDP-Ga_Gbg assoc—1e-06 | Ga-GTP_Gbg dissoc—10 | ||
| gabg-gdp dissoc—1e-03 | ||||
| Ga2 | G alpha auto GTPase—3 | |||
| Ga2_Rasgap | Ga2-GDP_RasGAP inact assoc—0.01 | Ga2-GTP_RasGAP dissoc—2e-03 | Ga2-GTP_RasGAP activation—100 | |
| Ga2-GTP_RasGAP assoc—0.01 | Ga2-GDP_RasGAP inact dissoc—2e-03 | |||
| RasGAP_Ga2-GTP assoc—1e-06 | ||||
| Gbg_Ras | Gbg_Ras assoc—1e-06 | Gbg_Ras dissoc—1e-03 | Gbg_Ras activation—100 | |
| Ras_RasGAP | Ras_RasGAP assoc—1e-07 | Ras-GTP_RasGAP dissoc—0 | Ras-GTP_RasGAP activation—0 | |
| RasGAP_RasGTP assoc—0 | Ras_RasGAP dissoc—1e-09 | Ras_RasGAP deactivation—1e-03 | ||
| RasGDP_RasGAP inactive assoc—0 | RasGDP_RasGAP inactive dissoc—0 | |||
| RasGAP | RasGAP deactivation—0.06 | |||
| Ras | Ras auto GTPase—3 | |||
| Figure 4B. RasGAP is activated by Rasc | ||||
| Ligand_GPCR | Ligand_GPCR binding—10,000,000 | Ligand_act-GPCR dissoc—1 | GPCR-basal: 50 | |
| Receptor_Gabg | Receptor_Gabg binding—50,000 | rec act ga gdp dissoc—0.01 | GPCR activation—3 | Ga2_Gbg_3 basal: 50 |
| rec act ga gtp dissoc—10 | Ras-GDP: 50 | |||
| rec inact ga dissoc—10 |
RasGAP_basal: 1e-06 | |||
| Ga2_Gbg | GDP-Ga_Gbg assoc—1e-06 | Ga-GTP_Gbg dissoc—10 | ||
| gabg-gdp dissoc—1e-03 | ||||
| Ga2 | G alpha auto GTPase—3 | |||
| Ga2_Rasgap | Ga2-GDP_RasGAP inact assoc—0 | Ga2-GTP_RasGAP dissoc—0 | Ga2-GTP_RasGAP activation—0 | |
| Ga2-GTP_RasGAP assoc—0 | Ga2-GDP_RasGAP inact dissoc—0 | |||
| RasGAP_Ga2-GTP assoc—0 | ||||
| Gbg_Ras | Gbg_Ras assoc—1e-07 | Gbg_Ras dissoc—1e-03 | Gbg_Ras activation—1e-03 | |
| Ras_RasGAP | Ras_RasGAP assoc—1e-09 | Ras-GTP_RasGAP dissoc—1e-05 | Ras-GTP_RasGAP activation—100 | |
| RasGAP_RasGTP assoc—1e-06 | Ras_RasGAP dissoc—1e=04 | Ras_RasGAP deactivation—1e-09 | ||
| RasGDP_RasGAP inactive assoc—0.1 | RasGDP_RasGAP inactive dissoc—0.1 | |||
| RasGAP | RasGAP deactivation—0.03 |
Diffusion coefficients [m^2/s]. Member proteins: GPCR: 1.0e-15, Ras: 1.0e-13, Gβγ: 1.0e-13, and Gα 1.0e-13. All cytosolic components (such as RasGAP) 1.0 e-11.
The set of parameters for the model of Figure 4C in which RasGAP is activated by both Gα-GTP and Ras-GTP.
The set of parameters for the model of Figure 4A in which RasGAP is activated by Gα-GTP.
The set of parameters for the model of Figure 4B in which RasGAP is activated by Ras-GTP.