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Overexpression PPP2AC results in the dephosphorylation of TFEB. (A) HeLa cells transfected with a control vector or plasmid, which overexpresses PPP2AC (WT, D85N)-FLAG, were incubated for 1 d in the growth medium and then analyzed by immunoblot using the indicated antibodies. (B) Quantitation of phospho-TFEB signal intensities from immunoblots in A and normalized to total TFEB protein (mean ± SD; three independent experiments). **P < 0.01.
