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. 2022 Jul 2;13:3814. doi: 10.1038/s41467-022-31253-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Schematic diagram of the humidity-induced attraction behavioral assay. The yellow region represents 20% RH generated by saturated LiCl solution, and the green region denotes 70% RH produced by saturated NaCl solution. A total of 20–40 flies were used per assay. b Spatial distribution of control flies (left) and flies with the antennae removed (right) assayed in a 20% to 70% RH gradient. w¹¹¹⁸ flies were used as control flies. Control, n = 22 flies; Antenna ablation, n = 20. The yellow dashed circle denotes the area above 20% RH, and the green circle indicates the 70% RH region. Color intensity represents the cumulative time the flies spent on each pixel during 80–90 s after the onset of the assay. c The time course indicating the attraction indexes of control and antenna-ablated flies. Control, n = 14 assays; Antenna ablation, n = 10. d Spatial distribution of Or42b > ReaperHid (left) and Or42b > Kir2.1 (right) flies assayed under the 20% to 70% RH condition. Or42b > ReaperHid, n = 24 flies; Or42b > Kir2.1, n = 25. e The time course indicating the attraction indexes of flies carrying indicated transgenes. Or42b-Gal4, n = 12 assays; Or42b > ReaperHid, n = 7; Or42b > Kir2.1, n = 11. f Attraction indexes after inhibiting thermosensory neurons or Or42b-expressing neurons or both. n = 10, 7, 7, 9, 10, 8 assays. g Humidity-guided attraction to 70% RH in flies with removal of different neuron groups by crossing UAS-ReaperHid with the indicated Gal4 lines. n = 11, 8, 6, 8, 7, 6, 6, 6, 6, 7, 9, 7, 8, 7, 7 assays. Two-tailed unpaired t test for two groups, one-way ANOVA followed by Holm-Sidak’s test for multiple comparison. For (c), (e), data points are mean values and shaded area represents ± SEM. For (f), (g), data are mean ± SEM. Source data are provided as a Source Data file.