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. 2022 Jul 2;13:3814. doi: 10.1038/s41467-022-31253-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Plateau attraction indexes for several olfactory receptor and mechanosensitive channel mutants tested in the 20–70% RH condition. n = 10, 10, 11, 11, 8, 9, 7, 8, 8, 9 assays. One-way ANOVA followed by Dunnett’s test for multiple comparison with the control group. b Tmem63 > GFP-labeled and Or42b > RFP-labeled axons project to overlapping regions of the antennal lobe. White dashed lines indicate the border of DM1 glomerulus and antennal lobe. Scale bar, 50 μm. Genotype: UAS-mCD8-RFP,LexAop2-mCD8-GFP; Tmem63^LexA/+; Or42b-Gal4/+. Representative images of three biological replicates. c Schematic illustration of in vivo calcium imaging in the antennal lobe. d Raw fluorescence image of Tmem63-expressing axons (in a Tmem63^LexA/+; LexAop2-GCaMP6m/+ animal) innervating the antennal lobe. Scale bar, 20 μm. e–g Pseudo color images showing GCaMP6m responses (ΔF/F₀) of Tmem63-expressing neurons to humidity stimulations or 1% apple cider vinegar (ACV). Scale bar, 20 μm. h ΔF/F₀ response to moist air (green) overlaid on ΔF/F₀ response to vinegar (magenta). Scale bar, 20 μm. d–h Representative images from over three independent replicated experiments. i Representative traces for moisture-activated response of DM1 glomerulus (upper) and dry air-activated response of VP4 glomerulus (lower) in different humidity changing cycles. j Peak ΔF/F₀ in response to humidity changes. n = 7 flies for each group. Data are mean ± SEM. Source data are provided as a Source Data file.