TABLE 2.
Expression of celY in E. coli DH5α using Sau3A1 fragments of Z. mobilis chromosomal DNA as surrogate promotersa
| Plasmids expressing celY or celZ | Endoglucanase activity
|
||
|---|---|---|---|
| Extracellular (IU/liter) | Total (IU/liter) | % Extracellular | |
| pMH18 (native celY promoter) | 151 | 184 | 82 |
| pLOI2317 (promoterless celY vector) | 0 | 0 | 0 |
| celY expressed from surrogate promoters | |||
| pLOI2318 | 1,123 | 1,257 | 89 |
| pLOI2319 | 888 | 1,023 | 87 |
| pLOI2320 | 1,023 | 1,056 | 97 |
| pLOI2323 | 1,257 | 1,291 | 97 |
| pLOI2342 | 1,224 | 1,257 | 97 |
| pLOI2349 (celZ) | 3,414 | 16,234 | 21 |
a
All plasmids are pUC derivatives. Endoglucanase activity was measured using cultures grown at 37°C for approximately 16 h.