TABLE 3.
Transcriptional initiation sites and putative promoter regions for the celY promoter in DH5α(pLOI2323)a
| Sequenceb | RNA start | Proposed ς factors | ς Factor consensus sequence
|
|
|---|---|---|---|---|
| −35 | −10 | |||
| ATATTTTTGATTTTTCAAGAAAAGCCTGATATCTTCCAACATCTT | T | ς70 | TTGACA | TATAAT |
| GATTTGATCCTCTAGAGTCAACCTGCTTGTTACTCGTGATCCCAT | A | ς70 | TTGACA | TATAAT |
| GAGTCAACCTGCTTGTTACTCGTGATCCCATTCACAAGGGCGAA | C | ς32 | CTTGAAA | CCCCAT |
| TTACTCGTGATCCCATTCACAAGGGCGAATTAATTCGCCCTT | C | ς38 | CCGCCT | TATACT |
Transcriptional starts for celY were identified by primer extension analysis. Four putative promoters are shown.
Upstream regions with similarity to E. coli −35 (left) and −10 (right) regions are underlined. RNA start sites are shown in boldface.