TABLE 4.
Effect of out genes (pCPP2006) on endoglucanase production by derivatives of K. oxytoca P2
| Strain or Spezyme additive | CMC zonea (mm) | OD550b | CMCase activityc
|
||
|---|---|---|---|---|---|
| Extracellular (IU/liter) | Total (IU/liter) | Secretion (%) | |||
| P2 | 0 | 10.5 | 0 | 0 | 0 |
| SZ6 | 8.5 | 11.0 | 1,920 | 8,800 | 22 |
| SZ21 | 6.7 | 11.0 | 1,620 | 7,800 | 21 |
| SZ22 | 2.0 | 10.0 | 480 | 879 | 55 |
| P2(pCPP2006) | 0 | 10.0 | 0 | 0 | 0 |
| SZ6(pCPP2006) | 10.8 | 9.6 | 13,800 | 22,300 | 62 |
| SZ21(pCPP2006) | 11.5 | 10.2 | 20,100 | 26,900 | 75 |
| SZ22(pCPP2006) | 2.0 | 9.7 | 449 | 833 | 54 |
| Spezyme CE (10 ml/liter)d | 27,000 | ||||
| Spezyme CP (10 ml/liter)d | 33,400 | ||||
a
Diameter of cleared zone on CMC indicator plates.
b
Culture density after 24 h of incubation (30°C in LB medium containing 5% sorbitol).
c
Endoglucanase activity was measured using cultures grown for 24 h.
d
Dilution equivalent to the highest Spezyme level used in fermentation experiments (Table 5).