TABLE 5.
Ethanol production from Sigmacell 50 (100 g/liter)
| Strain | Genencor Spezyme
|
Fermentationa
|
|||
|---|---|---|---|---|---|
| Type | Addition (ml/liter) | n | Ethanol concn ± SD (g/liter)b | % Control ± SD | |
| P2(pCPP2006) | None | 0 | 3 | 0.23 ± 0.01 | 100 ± 2.0 |
| SZ6(pCPP2006) | None | 0 | 3 | 0.28 ± 0.02* | 124 ± 8.5 |
| SZ21(pCPP2006) | None | 0 | 3 | 0.26 ± 0.02* | 116 ± 8.5 |
| SZ22(pCPP2006) | None | 0 | 3 | 0.24 ± 0.01 | 107 ± 1.0 |
| P2(pCPP2006) | CE | 5.0 | 6 | 13.7 ± 0.3 | 100 ± 2.0 |
| SZ6(pCPP2006) | CE | 5.0 | 6 | 13.8 ± 0.3 | 101 ± 2.4 |
| SZ21(pCPP2006) | CE | 5.0 | 6 | 16.0 ± 0.5** | 117 ± 3.5 |
| SZ22(pCPP2006) | CE | 5.0 | 6 | 15.2 ± 0.3** | 112 ± 1.5 |
| P2(pCPP2006) | CE | 10.0 | 6 | 20.7 ± 0.5 | 100 ± 2.1 |
| SZ6(pCPP2006) | CE | 10.0 | 6 | 21.2 ± 0.1 | 103.4 ± 0.4 |
| SZ21(pCPP2006) | CE | 10.0 | 6 | 24.6 ± 0.5** | 121 ± 2.3 |
| SZ22(pCPP2006) | CE | 10.0 | 6 | 25.2 ± 1.1** | 122 ± 5.0 |
| P2(pCPP2006) | CP | 5.0 | 6 | 15.2 ± 0.3 | 100 ± 1.7 |
| SZ21(pCPP2006) | CP | 5.0 | 6 | 17.8 ± 0.4** | 116 ± 2.2 |
| P2(pCPP2006) | CP | 10.0 | 6 | 25.3 ± 0.7 | 100 ± 2.6 |
| SZ21(pCPP2006) | CP | 10.0 | 6 | 27.2 ± 0.3** | 107 ± 1.2 |
a
Cultures grown without added cellulose or Spezyme produced 0.22 ± 0.01 g of ethanol per liter. Spezyme contained approximately 100 FPU/ml. The addition of 5 or 10 ml of Spezyme corresponds to 5 and 10 FPU/g of cellulose, respectively.
b
Student t test shows that there is significant difference in ethanol production compared to the respective P2 controls at each Spezyme dilution. A P value of ≤0.001 is indicated by two asterisks; a P value of ≤0.05 is indicated by one asterisk.