Table 3.
KEGG pathway overrepresentation analysis of ME2 module and list of involved genes in THP-1 macrophages co-cultured with MMNK-1 cholangiocytes.
| Gene set | Description | Size | Expected | Observed | Ratio | p-value | FDR |
|---|---|---|---|---|---|---|---|
| hsa04110 | Cell cycle | 124 | 1.63 | 9 | 5.53 | 3.3E−05 | 1.1E−02 |
| Genes involved in cell cycle | Regulation | p-value | q-value | Mean ± SD of normalized values fold change | |||
|---|---|---|---|---|---|---|---|
| 1,2-DCP concentration | |||||||
| 0 | 0.1 | 0.4 mM | |||||
| BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B) | Upregulated | 6.9E−05 | 0.02 |
56 ± 10 1 |
89.1 ± 5.8 1.60 |
110 ± 30 1.98 |
|
| Cyclin B2 (CCNB2) | Upregulated | 9.8E−04 | 0.08 |
36.1 ± 7.9 1 |
53.6 ± 4.9 1.49 |
70 ± 15 1.94 |
|
| Cell division cycle 20 (CDC20) | Upregulated | 6.6E−04 | 0.21 |
164 ± 11 1 |
224 ± 41 1.37 |
233 ± 50 1.42 |
|
| Cell division cycle 45 (CDC45) | Upregulated | 3.5E−02 | 0.42 |
64.1 ± 8.6 1 |
92.1 ± 5.6 1.44 |
76 ± 16 1.19 |
|
| Cell division cycle 7 (CDC7) | Upregulated | 6.9E−03 | 0.21 |
15.8 ± 4.6 1 |
29.6 ± 5.0 1.87 |
30.9 ± 1.0 1.95 |
|
| Cyclin dependent kinase 1 (CDK1) | Upregulated | 1.2E−06 | 7.69E−04 |
162 ± 14 1 |
240 ± 17 1.48 |
248 ± 15 1.53 |
|
| Minichromosome maintenance complex component 3(MCM3) | Upregulated | 6.4E−03 | 0.21 |
166 ± 43 1 |
229 ± 24 1.38 |
234 ± 19 1.41 |
|
| Polo like kinase 1 (PLK1) | Upregulated | 4.7E−02 | 0.46 |
40.5 ± 5.5 1 |
54.7 ± 4.6 1.35 |
60.0 ± 9.3 1.48 |
|
| Pituitary tumor-transforming 1 (PTTG1) | Upregulated | 2.5E−03 | 0.14 |
162 ± 15 1 |
236 ± 42 1.46 |
212 ± 32 1.31 |
|
The table shows KEGG pathway overrepresentation analysis of ME2 module and involved genes from the transcriptomic profiles of THP-1 macrophages co-cultured with MMNK-1 cholangiocytes when exposed to 1,2-DCP at 0, 0.1 or 0.4 mM for 24 h. The analysis was performed by WebGestalt with False Discovery Rate (FDR) cut-off value of 0.05. Data of normalized values for gene expression are mean ± SD, n = 3. All p-values for gene expression were adjusted using Benjamini–Hochberg method and expressed as q-values. Fold change represents the value relative to the average of the control group (0 mM).