Table 4.
Genes whose expression changed dose-dependently following exposure to 1,2-DCP in MMNK-1 cholangiocytes co-cultured with THP-1 macrophages.
| Genes | p-value | q-value | Mean ± SD of normalized values fold change | ||
|---|---|---|---|---|---|
| 1,2-DCP concentration | |||||
| 0 | 0.1 | 0.4 mM | |||
| Upregulated | |||||
| Aldehyde dehydrogenase 2 family (ALDH2) | 4.2E−05 | 4.5E−02 |
7.4 ± 2.3 1 |
7.5 ± 2.4 1.01 |
18.2 ± 2.3 2.27 |
| Phosphodiesterase 7B(PDE7B) | 3.9E−05 | 4.4E−02 |
10.6 ± 1.7 1 |
15.7 ± 5.0 1.48 |
25.0 ± 1.7 2.36 |
| DNA Ligase 1 (LIG1) | 1.6E−05 | 2.8E−02 |
157 ± 13 1 |
175 ± 15 1.12 |
213.0 ± 3.4 1.36 |
| Fibronectin 1 (FN1) | 1.9E−05 | 2.9E−02 |
338.0 ± 5.3 1 |
375 ± 36 1.11 |
458 ± 24 1.35 |
| General transcription factor IIE subunit 2 (GTF2E2) | 5.2E−05 | 4.9E−02 |
63.4 ± 7.3 1 |
67.1 ± 4.0 1.06 |
85.5 ± 4.4 1.35 |
| Downregulated | |||||
| Small nucleolar RNA, C/D box 32A (SNORD32A) | 1.1E−05 | 2.2E−02 |
6.2 ± 1.7 1 |
4.16 ± 0.78 0.68 |
0.31 ± 0.53 0.05 |
| Histone cluster 3 H2B family member b (HIST3H2BB) | 5.6E−05 | 4.9E−02 |
4.7 ± 1.3 1 |
4.57 ± 0.75 0.98 |
0.69 ± 0.61 0.15 |
| Ectonucleoside triphosphate diphosphohydrolase 5 (ENTPD5) | 5.8E−05 | 4.9E−02 |
56.9 ± 4.2 1 |
55.8 ± 8.3 0.98 |
32.1 ± 4.0 0.57 |
| Shugoshin 2 (SGO2) | 8.6E−06 | 1.9E−02 |
442 ± 19 1 |
415 ± 27 0.94 |
339 ± 12 0.77 |
| Nucleic acid binding protein 2 (NABP2) | 3.6E−05 | 4.3E−02 |
486 ± 17 1 |
467.3 ± 5.6 0.96 |
434.0 ± 8.3 0.89 |
We tested the significance of Pearson correlation coefficient between the expression of each gene and 1,2-DCP level.
The table lists the top or bottom five genes with the largest fold change at 0.4 mM in MMNK-1 cholangiocytes co-cultured with THP-1 macrophages when exposed to 1,2-DCP at 0, 0.1 or 0.4 mM for 24 h. Data of normalized values for gene expression are mean ± SD, n = 3. All p-values for gene expression were adjusted using Benjamini–Hochberg method and expressed as q-values. Fold change represents the value relative to the average of the control group (0 mM).