Table 5.
Genes whose expression changed dose-dependently following exposure of 1,2-DCP to THP-1 macrophages co-cultured with MMNK-1 cholangiocytes.
| Genes | p-value | q-value | Mean ± SD of normalized values fold change | ||
|---|---|---|---|---|---|
| 1,2-DCP concentration | |||||
| 0 | 0.1 | 0.4 mM | |||
| Upregulated | |||||
| Guanylate Cyclase 1 Soluble Subunit Beta 1 (GUCY1B1) | 1.5E−04 | 4.6E−02 |
0.31 ± 0.54 1 |
2.8 ± 1.1 8.87 |
4.9 ± 1.0 15.62 |
| CD48 molecule (CD48) | 1.7E−05 | 2.1E−02 |
0.33 ± 0.58 1 |
0.38 ± 0.65 1.12 |
3.12 ± 0.29 9.33 |
| unc-51 like kinase 4 (ULK4) | 2.2E−05 | 2.3E−02 |
4.00 ± 0.88 1 |
8.3 ± 4.5 2.06 |
17.5 ± 2.0 4.37 |
| killer cell lectin like receptor C1 (KLRC1) | 5.5E−06 | 1.0E−02 |
1.6 ± 1.0 1 |
3.23 ± 0.14 1.98 |
5.50 ± 0.07 3.37 |
| regulator of G-protein signaling 13 (RGS13) | 1.6E−04 | 4.8E−02 |
5.6 ± 4.0 1 |
4.2 ± 2.6 0.75 |
17.6 ± 1.1 3.13 |
| Downregulated | |||||
| solute carrier family 5 member 12 (SLC5A12) | 9.7E−05 | 4.0E−02 |
6.4 ± 1.9 1 |
4.0 ± 1.4 0.62 |
0.37 ± 0.64 0.06 |
| Prader Willi/Angelman region RNA 6 (PWAR6) | 4.0E−05 | 2.8E−02 |
2.01 ± 0.14 1 |
1.98 ± 0.34 0.98 |
0.31 ± 0.54 0.15 |
| DAB1, reelin adaptor protein (DAB1) | 4.1E−05 | 2.7E−02 |
5.67 ± 0.32 1 |
5.2 ± 1.9 0.91 |
1.04 ± 0.10 0.18 |
| Argonaute 4, RISC catalytic component (AGO4) | 2.3E−05 | 2.2E−02 |
18.7 ± 2.1 1 |
17.4 ± 4.5 0.93 |
4.8 ± 1.7 0.26 |
| Intercellular adhesion molecule 5 (ICAM5) | 1.5E−04 | 4.6E−02 |
9.7 ± 1.6 1 |
8.6 ± 2.6 0.89 |
2.8 ± 1.4 0.29 |
We tested the significance of Pearson correlation coefficient between the expression of each gene and 1,2-DCP level.
The table lists the top or bottom five genes with the largest fold change at 0.4 mM in THP-1 macrophages co-cultured with MMNK-1 cholangiocytes when exposed to 1,2-DCP at 0, 0.1 or 0.4 mM for 24 h. Data of normalized values for gene expression are mean ± SD, n = 3. All p-values for gene expression were adjusted using Benjamini–Hochberg method and expressed as q- values. Fold change represents the value relative to the average of the control group (0 mM).