Table 1.

Characterization and validation.

Classification	Test	Result	Data
Morphology	Photography bright field	Normal	Fig. 1A
Phenotype	Qualitative analysis: Immunofluorescence staining	Positive expression of pluripotency markers: Oct3/4, Nanog, and Sox2	Fig. 1B
	Quantitative analysis: RT-qPCR	Positive expression of NANOG and SOX2 in iPSCs; negative in differentiated CMs	Fig. 1D
Genotype	Whole genome array (Karyostat™ Assay) Resolution 1–2 Mb	Normal karyotype: 46, XY for SCVIi-039 and SCVIi-040, XX for SCVIi-041	Supplementary Fig. 1A
Identity	Microsatellite PCR (mPCR) or STR analysis	mPCR not performed	N/A
		16 loci tested with matching identity	Supplementary Fig. 2A,B; Also submitted in archive with journal
Mutation analysis (IF APPLICABLE)	Sequencing	Heterozygous for all three lines	Fig. 1F
	Southern blot or WGS	N/A	N/A
Microbiology and virology	Mycoplasma	Luminescence: negative	Supplementary Fig. 1B
Differentiation potential	Trilineage in vitro differentiation	Positive IF staining of the three germ layers	Fig. 1C
List of recommended germ layer markers	Expression of these markers has to be demonstrated at mRNA (RT-qPCR) or protein (IF) levels, at least 2 markers need to be shown per germ layer	Ectoderm: Otx2, Pax6; Mesoderm: Brachyury, Tbx6; Endoderm: Sox17, Foxa2	Fig. 1C
Donor screening (OPTIONAL)	HIV 1 + 2, Hepatitis B, Hepatitis C	Not performed	N/A
Genotype additional info (OPTIONAL)	Blood group genotyping	Not performed	N/A
	HLA tissue typing	Not performed	N/A