Table 2.
Reagents details.
| Antibodies used for immunocytochemistry/flow-cytometry | ||||
|---|---|---|---|---|
| Antibody | Dilution | Company Cat # | RRID | |
| Pluripotency marker | Rabbit Anti-Nanog | 1:200 | Protein tech Cat# 142951-1-AP | AB_1607719 |
| Pluripotency marker | Mouse IgG2bκ Anti-Oct-3/4 | 1:200 | Santa Cruz Biotechnology Cat# sc-5279 | AB_628051 |
| Pluripotency marker | Mouse IgG1κ Anti-Sox2 | 1:200 | Santa Cruz Biotechnology Cat# sc-365823 | AB_10842165 |
| Ectoderm marker | Goat Anti-Otx2 | 1:200 | R&D Systems Cat# 963273 | AB_2157172 |
| Ectoderm marker | Rabbit Anti-Pax6 | 1:100 | Thermo Fisher Scientific Cat # 42-6600 | AB_2533534 |
| Mesoderm marker | Goat Anti-Brachyury | 1:200 | R&D Systems Cat# 963427 | AB_2200235 |
| Mesoderm marker | Rabbit Anti-Tbx6 | 1:200 | Thermo Fisher Scientific Cat# PA5-35102 | AB_2552412 |
| Endoderm marker | Goat Anti-Sox17 | 1:200 | R&D Systems Cat# 963121 | AB_355060 |
| Endoderm marker | Rabbit Anti-Foxa2 | 1:250 | Thermo Fisher Scientific Cat# 701698 | AB_2576439 |
| Secondary antibody | Alexa Flour 488 Goat Anti-Mouse IgG1 | 1:1000 | Thermo Fisher Scientific Cat#A-21121 | AB_2535764 |
| Secondary antibody | Alexa Flour 488 Donkey Anti-Goat IgG (H + L) | 1:1000 | Thermo Fisher Scientific Cat#A-11055 | AB_2534102 |
| Secondary antibody | Alexa Fluor 555 Goat Anti-Rabbit IgG (H + L) | 1:500 | Thermo Fisher Scientific Cat#A-21428 | AB_141784 |
| Secondary antibody | Alexa Fluor 647 Goat Anti-Mouse IgG2B | 1:250 | Thermo Fisher Scientific Cat#A-21242 | AB_2535811 |
| Secondary antibody | Alexa Fluor 555 Donkey Anti-Rabbit IgG (H + L) | 1:500 | Thermo Fisher Scientific Cat#A-31572 | AB_2180682 |
| Primers Target | Size of band | Forward/Reverse primer (5′−3′) | ||
| Sendai virus plasmid (qPCR) | Sendai virus genome | 181 bp | Mr04269880_mr | |
| Genotyping | LMNA: c.398 G > A | 280 bp | F: CTGACCTCCTGGGAGCCT R: CATGTGTTAGGTGGGGCCAT |
|
| House-keeping gene (qPCR) | GAPDH | 471 bp | HS02786624_g1 | |
| Pluripotency genes (qPCR) | SOX2 | 258 bp | HS04234836_s1 | |
| NANOG | 327 bp | HS02387400_g1 | ||