Table 3.

List of primers with Tm used for amplification of different parts of PAL gene (Thiyagarajan et al. 2016)

S. no	Primer	Target buckwheat species	Sequence	Tm
1	FT E1 Start F FT E2 Nr R	F. tataricum	[F]ATGGGGGTCTCAAACGGA [R]TCGCCAGAAGCAGTGATG	56 °C
2	FT E1 VFr F FT E2AMed R	F. tataricum	[F] TAAGGAAGGCGGTGCTCTT [R]GCTTCTGAGAGGATCGAGTTC	55 °C
3	FT E2 BMed F FT E2 End R	F. tataricum	[F]GAGCTTCACGAACTCGATCC [R]CTAGCAGATAGGCAGAGGAGCA	56 °C