. Author manuscript; available in PMC: 2023 Jul 1.

Published in final edited form as: Pharm Res. 2022 Feb 15;39(7):1523–1534. doi: 10.1007/s11095-022-03186-1

Table 2.

Pharmacokinetic analysis of ³H-2-PAM after IV injection of ³H-2-PAM@scFv-Lipo in mice.

Organs	V_d-V₀ (μL/g)		PS (μL/min/g)		%ID/g
Organs	³H-2PAM@46.1-Lipo	³H-2PAM@Ctrl-Lipo	³H-2PAM@46.1-Lipo	³H-2PAM@Ctrl-Lipo	³H-2PAM@46.1-Lipo	³H-2PAM@Ctrl-Lipo
brain	5.39 ± 0.35^*	0.82 ± 1.43	0.016 ± 0.0036^*	0.0024 ± 0.0041	0.24 ± 0.016^*	0.023 ± 0.040
heart	32.36 ± 2.58	41.86 ± 9.91	0.097 ± 0.023	0.10 ± 0.045	1.43 ± 0.11	1.17 ± 0.28
lung	21.32 ± 1.93	31.46 ± 10.60	0.063 ± 0.010	0.076 ± 0.036	0.94 ± 0.086	0.88 ± 0.30
liver	67.60 ± 10.03^*	162.80 ± 11.56	0.20 ± 0.039	0.39± 0.13	2.99 ± 0.44^*	4.55 ± 0.32
spleen	38.93 ± 4.85^*	108.52 ± 14.24	0.12 ± 0.026^*	0.25± 0.040	1.72 ± 0.21^*	3.03 ± 0.40
kidney	27.72 ± 3.40^*	53.63 ± 8.04	0.081 ± 0.0093	0.12 ± 0.026	1.23 ± 0.15	1.50 ± 0.22

³H-2-PAM@scFv-Lipo was intravenously administered to C57BL6 mice at a dose corresponding to 5 mg/kg of liposomally-conjugated scFvs, and 3.4–4.3 × 10⁷ DPM/kg of ³H-2-PAM (n=3). An aliquot of orbital vein blood sample was collected at 0.5, 2, 15, 30, 60, 120 and 240 min after injection. Plasma amounts of ³H-2-PAM were determined by scintillation counting and used to determine the AUC. After 4 hours of circulation, mice were whole body perfused, organs were isolated, and scintillation counting was used to measure 2-PAM uptake in the whole organ homogenates. Organ uptake parameters were calculated as described in the Materials and Methods. Reported are means ± S.D., n=3 independent mice.

p<0.05 by two-tailed unpaired Student’s t test.