Qtw, H+, Pi, and H2PO4− were measured using electric femoral nerve stimulation and phosphorus magnetic resonance spectroscopy (31P-MRS) during 60 knee-extensor MVCs. Trial 1 and 2 were separated by 5 min of rest. Individual participant data are presented in panels A,C, & E (these data are fit with linear regressions for illustrative purposes only). The linear mixed effects model results on the standardized (Z-score) Qtw, H+, Pi, and H2PO4− are presented in panels B, D, & F. N = 10.