Table 1. Plasma cytokine evaluation of Triangle and Horse DNA origami nanostructures in vivo.

Female ICR mice underwent a repeat dosing regimen with treatment groups described in Figure 3A. Plasma isolated from peripheral blood collected on days 0, 1, 3, 5, and 10 was analyzed for a panel of cytokine/chemokine levels including inflammatory mediators (IFN-γ, IL-1β, IL-6, IL-10, IL-12(p70), TNF-α) chemoattractants (MCP-1, MIP-2, MIG) and T-cell cell mediators (IL-2, IL-5, IL-17); (IL-4 and IL-9 are shown in Tables S1–S2) via the Milliplex Magnetic Cytokine array.

The data are expressed as a heat map mean Fold Change relative to day 0 for each cytokine/chemokine: gray (0.0–1.0, little to no change); yellow (1.01–2.0, mild response); orange (2.01–4.0, intermediate response); red (>4.01, strong response).