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Antibodies/Flow Cytometry Dyes
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| Anti-mouse CD8a BUV395 (53-6.7) |
BD Biosciences |
Cat# 563786, RRID: AB_2732919 |
| Anti-mouse CD8a BUV496 (53-6.7) |
BD Biosciences |
Cat# 750024,, RRID: AB_2874242 |
| Anti-Mouse CD45.1 violetFluor 450 (A20) |
Tonbo Biosciences |
Cat# 75-0453, RRID:AB_2621949 |
| Anti-Mouse CD45.1 BUV395 (A20) |
BD Biosciences |
Cat# 565212, RRID:AB_2722493 |
| Anti-Mouse CD45.2 violetFluor 450 (104) |
Tonbo Biosciences |
Cat# 75-0454, RRID:AB_2621950 |
| Anti-Mouse CD45.2 BUV737 (104) |
BD Biosciences |
Cat# 564880, RRID:AB_2738998 |
| Anti-Mouse CD45.2 FITC (104) |
Tonbo Biosciences |
Cat# 35-0454, RRID:AB_2621692 |
| Anti-mouse CD44 BV786 (IM7) |
BD Biosciences |
Cat# 563736, RRID:AB_2738395 |
| Anti-mouse CD44 AF700 (IM7) |
Thermo Fisher Scientific |
Cat# 56-0441-82 RRID:AB_494011 |
| Anti-Mouse CD69 PE-Cy7 (H1.2F3) |
BD Biosciences |
Cat# 552879, RRID:AB_394508 |
| Anti-Mouse CD69 BV605 (H1.2F3) |
BD Biosciences |
Cat# 104529, RRID:AB_11203710 |
| Anti-mouse CD103 BV510 (M290) |
BD Biosciences |
Cat# 563087, RRID:AB_2721775 |
| Anti-mouse P2X7 PE (1F11) |
BD Biosciences |
Cat# 565345, RRID:AB_2739198 |
| Anti-mouse P2X7R APC (1F11) |
BioLegend |
Cat# 148706, RRID:AB_2650954 |
| Anti-mouse CD279 (PD-1) (RMP1-130) |
BioLegend |
Cat# 109119, RRID:AB_2566640 |
| Anti-Mouse CD279 (PD-1) (RMP1-130) |
BD Biosciences |
Cat# 748264, RRID:AB_2872692 |
| Anti-Mouse CD39 PerCP-eFluor 710 (24DMS1) |
Thermo Fisher Scientific |
Cat# 46-0391-82, RRID:AB_10717953 |
| Anti-Mouse CD223 (LAG-3) PE/Dazzle 594 (C9B7W) |
BioLegend |
Cat# 125224, RRID:AB_2572082 |
| Anti-Mouse CD366 (Tim-3) BV421 (RMT3-23) |
BioLegend |
Cat# 119723, RRID:AB_2616908 |
| Anti-Mouse TCF1/7 PE (C63/D9) |
Cell Signaling Technologies |
Cat# 14456S RRID: AB_10828102 |
| Anti-Mouse TOX eFluor 660 (TXRX10) |
Thermo Fisher Scientific |
Cat# 50-6502-82 RRID: AB_2574265 |
| Anti-Mouse Ki-67 FITC (SolA15) |
Thermo Fisher Scientific |
Cat# 11-5698-82 RRID: AB_11151330 |
| Anti-Mouse IFN-γ PE-Cy7 (XMG1.2) |
BioLegend |
Cat# 505826 RRID: AB_2295770 |
| Anti-Mouse Granzyme B PE (GB11) |
Thermo Fisher Scientific |
Cat# 12-8899-41 RRID: AB_1659718 |
| Annexin V APC |
BD Biosciences |
Cat# 550475 RRID: AB_2868885 |
| Ghost Dye Red 780 Viability Dye |
Tonbo Biosciences |
Cat # 13-0865-T100 |
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| MitoTracker Green FM |
Thermo Fisher Scientific |
Cat # M7514 |
| MitoSOX Red Mitochondrial Superoxide Indicator |
Thermo Fisher Scientific |
Cat # M36008
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| Tetramethylrhodamine, Ethyl Ester, Perchlorate (TMRE) |
Thermo Fisher Scientific |
Cat # T669 |
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| CellTrace Far Red Cell Proliferation Kit |
Thermo Fisher Scientific |
Cat# C34564
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| InCucyte Caspase 3/7 Dye for Apoptosis |
Sartorius |
Cat# 4440 |
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| InVivoMAb anti-mouse CD3ε (145-2C11) |
BioXCell |
Cat# BE0001-1, RRID:AB_1107634 |
| InVivoMAb anti-mouse CD28 (37.51) |
BioXCell |
Cat# BE0015-1, RRID:AB_1107624 |
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Chemicals, peptides, and recombinant proteins
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| Collagenase, Type 1 |
Worthington |
Cat# LS004197
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| Dithioerythritol |
EMD Millipore |
Cat# 233152-5GM |
| RPMI 1640 |
Corning |
Cat# 10-040-CV |
| HBSS 10x |
Corning |
Cat# 20-021-CV |
| Fetal Bovine Serum |
Atlas Biologicals |
Cat# FS-0500-AD |
| L-Glutamine, 100x, Liquid |
Corning |
Cat# 25-005-CI |
| Penicillin/Streptomycin |
Gibco |
Cat# 15070063 |
| DMEM |
Gibco |
Cat# 11995065 |
| A-438079 |
Abcam |
Cat# ab120413 |
| Human recombinant IL-2 |
Peprotech |
Cat# 200-02 |
| Mouse recombinant IL-12 |
R&D Systems |
Cat# 419-ML-050/CF |
| Human recombinant IL-12 |
R&D Systems |
Cat# 219-IL-005/CF |
| 2’(3’)-O-(4-Benzoylbenzoyl) adenosine 5’-triphosphate triethylammonium salt (Bz-ATP) |
Sigma-Aldrich |
Cat# B6396-25MG |
| XF Base Medium Minimal DMEM |
Agilent Technologies |
Cat# 102353-100 |
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Critical commercial assays
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| Naïve CD8a+ T cell Isolation Kit, Mouse |
Miltenyi Biotec |
Cat# 130-096-543 |
| BD Cytofix/Cytoperm Solution Kit |
BD Biosciences |
Cat#554714 |
| FoxP3/Transcription Factor Staining Buffer Set |
Tombo Bioscience |
Cat# TNB-0607-KIT |
| Naive CD8a+ T cell Isolation Kit, Human |
Stem Cell Technologies |
Cat# 19258 |
| Seahorse XF Cell Mito Stress Test Kit |
Agilent Technologies |
Cat# 103015-100 |
| QiaShredder Columns |
Qiagen |
Cat# 79654 |
| RNeasy Plus Mini Kit |
Qiagen |
Cat# 74134 |
| SuperScript III First-Strand Synthesis SuperMix |
Invitrogen |
Cat# 11752050 |
| SYBR Green PCR Master Mix |
Thermo Fisher Scientific |
Cat# 4309155 |
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Experimental models: Cell lines
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| B16.OVA Murine Melanoma |
Dr. M. Mescher, University of Minnesota |
N/A |
| B16.gp33 Murine Melanoma |
Dr. A. Goldrath, University of San Diego |
N/A |
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Experimental models: Organisms/strains
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| Mouse: B6.SJL-PtprcaPepcb/BoyCrCrl (B6-CD45.1) |
NCI Charles River |
Cat# CRL:564, RRID:IMSR_CRL:564 |
| Mouse: C57BL/6NCrl (B6-CD45.2) |
NCI Charles River |
Cat# CRL:027, RRID:IMSR_CRL:027 |
| Mouse: P14 |
Dr. R. Ahmed, Emory University |
N/A |
| Mouse: OT-I |
Dr. K. Hogquist, University of Minnesota |
N/A |
| Mouse: B6.129P2-P2rx7tm1Gab/J (P2rx7−/−) |
Jackson Labs |
Stock# 005576 |
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Oligonucleotides
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hP2rx7 Forward Primer: 5’- TGT GTC CCG AGT ATC CCA CC -3’ |
Integrated Data Technologies |
N/A |
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hP2rx7 Reverse Primer 5’- GGC ACT GTT CAA GAG AGC AG -3’ |
Integrated Data Technologies |
N/A |
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hGUSB Forward Primer 5’- GTC TGC GGC ATT TTG TCG -3’ |
Integrated Data Technologies |
N/A |
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hGUSB Reverse Primer 5’- CAC ACG ATG GCA TAG GAA TGG -3’ |
Integrated Data Technologies |
N/A |
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Software and algorithms
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| GraphPad Prism v9.2.0 |
GraphPad Software |
RRID:SCR_002798 |
| FlowJo v10.8 |
BD Biosciences |
RRID:SCR_008520 |
| BD FACSDiva |
BD Biosciences |
RRID:SCR_001456 |
| Seahorse Wave Desktop Software |
Agilent Technologies |
RRID: SCR_014526 |
