Figure 2.

PBZ treatment activates CsACS-mediated ET signal transduction in cucumber. (a) Heatmap of DEGs related to SA, ET, and JA biosynthesis/signaling in 4-hour PBZ-treated cucumber leaves (n = 2 biological replicates). (b) Expression time course of CsACS1, CsACS1-2, CsICS1, and CsPR1 in response to PBZ treatment. Primers used for qPCR are listed in Table S1; data are means ± SD (n = 3 biological replicates). (c) ET production in PBZ- and H₂O-treated cucumber. Data are means ± SD (n = 3 biological replicates); ^***P < 0.001 (t-test). (d) t-SNE visualization of transcriptomes from different treatments. Tissues were collected after a 30-minute treatment (n = 2 biological replicates). Mock treatment (control for PBZ): 0.1% (v/v) acetone solution containing 0.05% (w/v) Tween-20. (e–g) Volcano plots of the DEGs for 30-minute PBZ vs. mock (e), ACC vs. H₂O (f), and SA vs. H₂O (g). (h) Heatmap of DEGs in e–g. DEGs from PBZ vs. mock, ACC vs. H₂O, and SA vs. H₂O were combined.