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. 2022 Apr 22;9:uhac099. doi: 10.1093/hr/uhac099

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© The Author(s) 2022. Published by Oxford University Press on behalf of Nanjing Agricultural University

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Dual-luciferase (Dual-LUC) assays showed the suppression effect of OpWRKY6 on the promoters of camptothecin biosynthesis genes. a. Schematic diagrams of the effector and reporter plasmids used in Dual-LUC assays. REN, Renilla luciferase internal reference gene. LUC, firefly luciferase reporter gene. b. Dual-LUC assay in N. benthamiana leaf cells using the constructs shown in (a). The empty vector pHB-YFP was used as the control. The relative LUC activity was normalized to the reference Renilla (REN) luciferase. Error bars indicate the SD (n = 3). Student’s t-test: ^*p < 0.05.