TABLE 1.
PCR primers targeting the RPP classes
| Primer | Class targeted | Sequence | PCR annealing temp (°C) | Amplicon size (bp) |
|---|---|---|---|---|
| Ribo2-FWa | All except Otr A | GGMCAYRTGGATTTYWTIGC | Touchdownb | 1,187 |
| Ribo2-RV | TCIGMIGGIGTRCTIRCIGGRC | |||
| TetB/P-FW | TetB P | AAAACTTATTATATTATAGTG | 46 | 169 |
| TetB/P-RV | TGGAGTATCAATAATATTCAC | |||
| TetM-FW | Tet M | ACAGAAAGCTTATTATATAAC | 55 | 171 |
| TetM-RV | TGGCGTGTCTATGATGTTCAC | |||
| TetO-FW | Tet O | ACGGARAGTTTATTGTATACC | 60 | 171 |
| TetO-RV | TGGCGTATCTATAATGTTGAC | |||
| OTR-FW | Otr A | GGCATYCTGGCCCACGT | 66 | 212 |
| OTR-RV | CCCGGGGTGTCGTASAGG | |||
| TetQ-FW | Tet Q | AGAATCTGCTGTTTGCCAGTG | 63 | 169 |
| TetQ-RV | CGGAGTGTCAATGATATTGCA | |||
| TetS-FW | Tet S | GAAAGCTTACTATACAGTAGC | 50 | 169 |
| TetS-RV | AGGAGTATCTACAATATTTAC | |||
| TetT-FW | Tet T | AAGGTTTATTATATAAAAGTG | 46 | 169 |
| TetT-RV | AGGTGTATCTATGATATTTAC | |||
| TetW-FW | Tet W | GAGAGCCTGCTATATGCCAGC | 64 | 168 |
| TetW-RV | GGGCGTATCCACAATGTTAAC |
a
FW, forward; RV, reverse.
b
PCR conditions are described in Materials and Methods.