TABLE 2.
Characteristics of bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| Strains | ||
| C. jejuni subsp. jejuni | Tcr, tet(O) | ATCC 43503 |
| S. pyogenes A498 | Tcr, tet(T) | 9 |
| C. perfringens JIR4202 | Tcr, tetA(P) tetB(P) | 38 |
| Plasmids | ||
| pBT-1 | pJRD215 carrying a 2.5-kb SstI fragment containing the tet(Q) gene of TcrEmrDOT | 28 |
| pJIR667 | pPR328 carrying a 1.1-kb PstI-EcoRI internal fragment of the tetB(P) gene | 20 |
| pFD310 | Carries the tet(M) gene from S. agalactiae | 39 |
| pGEM-tetW | pGEM carrying a 2.4-kb PCR product with the tet(W) gene from B. fibrisolvens | 2 |
| pGEM-tetO | pGEM carrying the tet(O) gene from B. fibrisolvens | 2 |
| pVP2 | pUC18 carrying a 5.88-kb ClaI fragment of pK214 encompassing the tet(S) gene | 31 |
| pAT451 | pUC18 carrying a 4.5-kb ClaI fragment of pIP811 with the tet(S) gene | 7 |
| pCT10 | Contains the tet gene from S. lividans 1326 | 10 |