Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jul 2;23(1):75. doi: 10.1186/s10194-022-01442-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 5 — Inhibition of P2X7R attenuated IS-induced NLRP3 inflammasome activation and pyroptosis. (a-b) Representative immunoblots and quantification showed decreased expression levels of P2X7R, NLRP3 (inflammasome receptor) and cleaved caspase-1 (marker of inflammasome activation) in the cerebral cortex of IS-BBG mice compared to IS-VEH mice (n = 6 mice per group). (c-d) Representative immunoblots and quantification showed decreased expression levels of GSDMD-NT (pyroptotic marker) in the cerebral cortex of IS-BBG mice compared to IS-VEH mice (n = 6 mice per group). (e–f) ELISA results showed decreased release of IL-1β and IL-18 (direct downstream products of inflammasome activation) in the cerebral cortex of IS-BBG mice compared to IS-VEH mice (n = 4 mice per group). ^*p < 0.05, ^**p < 0.01 and ^***p < 0.001 as compared to sham-VEH mice; ⁺p < 0.05, ⁺⁺p < 0.01 and ⁺⁺⁺p < 0.001 as compared to IS-VEH mice. One-way ANOVA with Tukey’s multiple comparisons or Kruskal–Wallis with Dunn’s multiple comparisons test were performed for statistical analysis. Data are represented as mean ± SEM. Abbreviations: IS, inflammatory soup; BBG, brilliant blue G; VEH, vehicle; GSDMD-NT, N-terminal Gasdermin D; ELISA, enzyme-linked immunosorbent assay