Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jul 2;23(1):75. doi: 10.1186/s10194-022-01442-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 6 — Inhibition of P2X7R attenuated IS-induced NLRP3 inflammasome activation and pyroptosis on neurons and microglia in the cerebral cortex and hippocampus. (a, d) Double immunofluorescence staining showed a reduction in CC1-positive (marker of inflammasome activation) neurons (NeuN positive) and microglia (Iba-1 positive) in the cerebral cortex and hippocampus of IS-BBG mice compared to IS-VEH mice. (b, e) Double immunofluorescence showed similar CC3-positive (apoptotic marker) neurons (NeuN positive) in the cerebral cortex and hippocampus of the four groups of mice. (c, f) Double immunofluorescence staining showed a reduction in GSDMD-positive (pyroptotic marker) neurons (NeuN positive) and microglia (Iba-1 positive) in the cerebral cortex and hippocampus of IS-BBG mice compared to IS-VEH mice. Magnification × 100. Scale bar, 20 μm. Images were taken under identical exposures and conditions. Abbreviations: IS, inflammatory soup; CC1, cleaved caspase-1; CC3, cleaved caspase-3; GSDMD, Gasdermin D; NeuN, neuronal nuclei; Iba-1, ionized calcium-binding adaptor molecule-1; GFAP, glial fibrillary acidic protein; BBG, brilliant blue G; VEH, vehicle