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. 2022 Jul 2;23:484. doi: 10.1186/s12864-022-08721-3

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — Validation of the expression patterns for genes selected from RNA-seq analysis by realtime qRT-PCR. Gene expression values were obtained by normalizing the values. GAPDH was used as aninternal control. A Differences in gene expression levels among different treatments. B Differences in mean gene expression levels among different treatments. C The qRT-PCR data ratio of up-regulated DEGs of M9T337 (T) or M.26 (M) dataset. The color intensity was proportional to the normalizing the values. Taxa relative abundances were log10-transformed, and the scale method (from zero to one) was used for the heatmap representation. Each treatment included three repetitions