Figure 4.

EMSA with extracts of (A) C.thummi and (B) HeLa cells using an oligonucleotide containing telomeric HSE as probe. The C.thummi extracts used were from non-treated larvae (lane 1) and from larvae heat shocked for 2 h at 35°C (lanes 2–4). As competitors, the same non-labelled probes were used (lane 3) as well as an unlabelled oligonucleotide (HSE29C) covering the same region as the TsC repeat (lane 4), in 50-fold excess. HeLa extracts are from control cells (lane 1), infected with adenovirus, and from cells constitutively expressing HSF (lanes 2–4), infected with the adenovirus containing the HSF-1 gene. In (B) the extract in lane 3 was preincubated with a commercial antibody against HSF-1 and that in lane 4 was previously incubated with a preimmune serum from rabbit. F indicates free probe and arrows show HSF bands.