Table 1. Telomeric activation at different chromosome ends in C.thummi after 2 h of 35°C heat shock.

	Il	Ir	IIl	IIr	IIIl	IIIr	IVr
Puff size was measured as large (+++), medium (++), small (+) or not visible (–). Puffing frequency was considered high (H) when it reached >30%, medium (M) when between 10 and 30% and low when <10% (L). In telomere IVr no puffing was observed. Transcriptional activity was studied using an antibody against DNA–RNA hybrids. The frequency of telomeres with a positive signal (+, >50%; ++, >60%; +++, >80%) is indicated. HSF association indicates the presence (+) or absence (–) of a positive signal. At least five different larvae and 30–40 chromosomes per preparation were scored in each case.
Size of puff	+	++	–	–	–	+++	–
Puffing frequency	M	M	L	L	L	H	N
Transcriptional activity	++	++	++	++	+	+++	–
HSF association	–	+	–	–	–	+	–