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. 2022 Jul 3;25(8):104709. doi: 10.1016/j.isci.2022.104709

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© 2022 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Palmitoylation of WT and cysteine-rich cluster mutants S protein

(A) Palmitoylated proteins in 293T/17 cells were treated with 10 kDa methoxy polyethylene glycol maleimide (mPEG-mal) and immunoblotted with an anti-S2 antibody.

(B) Palmitoylated S protein in S_pp was labeled with 5 kDa mPEG-mal and detected by immunoblotting.

(C) Sequence of cysteine-rich motif of the WT and mutant S proteins.

(D) Palmitoylation of cysteine-rich cluster mutants as determined by acyl-PEG exchange.

(E) The S protein expression of WT and cysteine-rich cluster mutants in 293T/17 cells and S_pp was examined by immunoblotting with an anti-S2 antibody.

(F) The titers for WT and cysteine mutants S_pp were evaluated. Error bars represent SEM and n = 3. Red circle: S protein; blue circle: S2 subunit. +: number of different palmitoylated species.