Table 2.

Overview of binding and affinity of top eight mAbs with RBD mutant antigens

	mAb	1A10	1C1	1C11	2B11	2B2	2D6	3B8	3E6
ELISA antigen binding	Wuhan	+	+	+	+	+	+	+	+
	N439K	+	+	+	+	+	+	+	+
	L452R	+	+	+	±	+	+	+	+
	Y453F	+	+	+	+	+	+	+	+
	E484K	+	+	+	/	+	+	+	+
	E484Q	+	+	+	+	+	+	+	+
	N501Y	+	+	+	+	+	+	+	+
	L452R;E484Q	+	+	/	/	+	+	+	+
	K417N, E484K, N501Y	+	+	+	/	+	+	+	+
		1A10	1C1	1C11	2B11	2B2	2D6	3B8	3E6
Affinity KD(pM)	Wuhan	199 ± 58	41 ± 12	87 ± 24	48 ± 21	31 ± 7	31 ± 6	234 ± 104	373 ± 295
	N439K	379 ± 120	28 ± 14	107 ± 51	42 ± 13	16 ± 5	43 ± 51	144 ± 57	299 ± 295
	L452R	118 ± 19	9 ± 1	2940 ± 2020	3146 ± 737	145 ± 10	18 ± 1	56 ± 3	178 ± 75
	Y453F	170 ± 61	36 ± 18	142 ± 143	29 ± 12	23 ± 3	20 ± 6	136 ± 49	281 ± 173
	E484K	106 ± 33	23 ± 7	382 ± 116	–	450 ± 37	22 ± 7	119 ± 11	122 ± 86
	E484Q	101 ± 22	14 ± 3	167 ± 7	647 ± 347	174 ± 18	18 ± 2	105 ± 6	87 ± 49
	N501Y	151 ± 64	33 ± 19	118 ± 80	32 ± 17	19 ± 5	18 ± 5	159 ± 60	162 ± 159

Antigen binding was evaluated for RBD mutant antigens via ELISA and SPR. Affinity (K_D; in pM) was determined via SPR, mean ± SD of minimum three replicates is given. + = maximal signal obtained at mAb concentration of 1 μg/mL or lower; ± = ± 50% of the maximal signal was obtained at 1 μg/mL; / = no signal was obtained at mAb concentration of 1 μg/mL; - = no binding at RBD antigen concentration of 80 nM. See also Table S2.