REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
PerCP-cy5.5 anti-human CD19 antibody	Biolegend	Cat# 363016, clone SJ25C1; RRID:AB_2564207
FITC anti-human CD3 antibody	Biolegend	Cat# 300306, clone HIT3a; RRID:AB_314042
anti-SARS-CoV-2 RBD mAb	Sino Biological	Cat# 40150-D004; RRID:AB_2827983
anti-SARS-CoV-2 nucleocapsid antibody	MyBioSource	Cat# MBS2563841
Bacterial and virus strains
SARS-CoV-2 Wuhan (BetaCov/Belgium/GHB-03021/2020; EPI ISL 109 407976|2020-02-03)	Prof. Piet Maes; https://doi.org/10.1038/s41467-022-28354-0	PMID: 33037151
SARS-CoV-2 Beta B.1.351 (hCoV-19/Belgium/rega-1920/2021; EPI_ISL_896474, 2021-01-11)	Prof. Piet Maes; https://doi.org/10.1038/s41467-022-28354-0	PMID: 34049240
SARS-CoV-2 Delta B.1.617.2 (hCoV-19/Belgium/rega-7214/2021; EPI_ISL_2425097; 2021-04-20)	Prof. Piet Maes; https://doi.org/10.1038/s41467-022-28354-0	PMID: 35169114
Biological samples
Human COVID-19 patient PBMCs	This paper	N/A
Human COVID-19 patient serum	This paper	N/A
Chemicals, peptides, and recombinant proteins
PE streptavidin	Biolegend	Cat# 405203
Spike glycoprotein (S1) RBD-His	The Native Antigen Company	Cat# REC31849-500
RBD(N439K)-His	Sino Biological	Cat# 40592-V08H14
RBD(N501Y)-His	Sino Biological	Cat# 40592-V08H82
RBD(E484K)-His	Sino Biological	Cat# 40592-V08H84
RBD(Y453F)-His	Sino Biological	Cat# 40592-V08H80
RBD(E484Q)-His	Sino Biological	Cat# 40592-V08H81
RBD(L425R)-His	Sino Biological	Cat# 40592-V08H28
RBD(L425R,E484Q)-His	Sino Biological	Cat# 40592-V08H88
RBD(K417N, E484K, N501Y)-His	Sino Biological	Cat# 40592-V08H85
Spike Glycoprotein (Full-Length)-His	The Native Antigen Company	Cat# REC31868-500
Zombie Aqua Fixable Viability Kit	Biolegend	Cat# 423101
FcR blocking reagent	Miltenyi Biotec	Cat# 130-059-901
KAPA HiFi HotStart ReadyMix	Roche	Cat# 103568-586
Critical commercial assays
EasySep™ Human B Cell Enrichment Kit	Stem Cell Technologies	Cat# 17954
EZ-Link Sulfo-NHS-LC-Biotin kit	Thermo Fisher	Cat# 21335
Agencourt AMPure XP beads	Beckman Coulter	Cat# A63880
NucleoBond Xtra Maxi EF kit	Machery - Nagel	Cat# 740424.50
Experimental models: Cell lines
FreeStyle™ 293-F Cells	Thermo Fisher	Cat# R79007
HEK293T cells	ATCC	ATCC Cat# CRL-3216, RRID:CVCL_0063
Vero E6 cells	ATCC	ATCC Cat# CRL-1586
BHK-21J	https://doi.org/10.1128/JVI.71.12.96089617.1997	N/A
Experimental models: Organisms/strains
Golden Syrian Hamsters (Mesocricetus auratus)	Janvier Laboratories	Strain name RjHan:AURA
Oligonucleotides
oligo-dT primer (5′–AAGCAGT GGTATCAACGCAGAGTACT30-3′)	IDT DNA	N/A
Forward primer (described by Picelli et al.) (5′-AAGCAGTGGT ATCAACGCAGAGT-3′)	IDT DNA	N/A
Reverse primer IgM variable region (described by Ozawa et al.) (5′-CCGACGGGGAA TTCTCACAG-3′)	IDT DNA	N/A
Reverse primer IgG variable region (described by Ozawa et al.) (5′-CGCCTGAGTTCC ACGACACC-3′)	IDT DNA	N/A
Reverse primer Kappa variable region (described by Ozawa et al.) (5′-GAGGCAGTTCCA GATTTCAA-3′)	IDT DNA	N/A
Reverse primer Lamda variable region (described by Ozawa et al.) (5′-GCTT GGAGCTCCTCAGAGG-3′)	IDT DNA	N/A
Recombinant DNA
VSVΔG	https://doi.org/10.1016/j.jviromet.2010.08.006	N/A
D614G Spike variant	https://doi.org/10.1038/s41586-020-3035-9	N/A
Beta Spike variant	Invivogen	plv-spike-v3
Gamma Spike variant	Invivogen	plv-spike-v5
Delta Spike variant	Invivogen	plv-spike-v8
Omicron Spike variant	https://doi.org/10.1101/2021.11.12.468374	N/A
Software and algorithms
GraphPad Prism		N/A
Biacore T200 Evaluation Software 3.1		N/A
Thermo Fisher Scientific HCS Studio (v.6.6.0) software		N/A