Table 1.
Flow cytometry data of cell cultures
| Cells | FISH-TAMB probes |
Cell density (× 106 per mL) [mean] |
Cell density (× 106 per mL) [SD] |
No. of FISH-TAMB labeled cells# (106 per mL) [mean] |
No. of FISH-TAMB labeled cells# (106 per mL) [SD] |
% of FISH-TAMB labeled cells# [mean] |
% of FISH-TAMB labeled cells# [SD] |
MB probe (pmol per cell)^ [mean] |
MB probe (pmol per cell)^ [SD] |
|---|---|---|---|---|---|---|---|---|---|
| Uninduced E.coli mcrA + (n = 3) | mcrA | 0.340 | 0.036 | 0.000 | 0.000 | -0.01% | 0.03% | 2.96E-04 | 3.01E-05 |
| Uninduced E.coli mcrA + (n = 3) | 0.277 | 0.044 | 0.000 | 0.000 | 0.00% | 0.02% | 3.67E-04 | 5.41E-05 | |
| Induced E.coli mcrA + (n = 3) | mcrA | 3.805 | 2.274 | 3.602 | 2.363 | 92.48% | 5.65% | 3.40E-05 | 2.07E-05 |
| Induced E.coli mcrA + (n = 3) | 0.320 | 0.060 | 0.000 | 0.000 | 0.03% | 0.01% | 3.20E-04 | 6.06E-05 | |
| Induced E.coli mcrA + (n = 3) | lacZa | 0.325 | 0.083 | 0.000 | 0.000 | 0.01% | 0.02% | 3.20E-04 | 7.82E-05 |
| Uninduced E.coli lacZa + (n = 3) | lacZa | 0.276 | 0.112 | 0.155 | 0.082 | 54.13% | 7.19% | 4.09E-04 | 1.75E-04 |
| Induced E.coli lacZa + (n = 3) | lacZa | 0.879 | 0.386 | 0.638 | 0.253 | 74.15% | 7.04% | 1.33E-04 | 6.77E-05 |
| M. barkeri at exponential phase (n = 3) | mcrA | 0.023 | 0.010 | 0.006 | 0.002 | 28.83% | 4.76% | 4.70E-03 | 1.64E-03 |
| M. barkeri exposed to O2 (n = 3) | mcrA | 0.001 | 0.001 | 0.000 | 0.000 | 1.76% | 3.62% | 7.82E-02 | 3.67E-02 |
| ANME enrichment (n = 3) | mcrA | 0.049 | 0.015 | 0.001 | 0.000 | 2.62% | 0.19% | 2.05E-03 | 5.47E-04 |
#Background subtracted
^The amount of MB probes was calculated by dividing the cell density (counted by flow cytometry) by the amount of MB (in the form of FISH-TAMB) probes added to the reaction mixture
The result of uninduced and induced E. coli lacZa+ cells was described and discussed in the Supplementary Results and Discussion