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. 2022 Jun 20;13:875865. doi: 10.3389/fendo.2022.875865

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Copyright © 2022 Lopez Ruiz, Ernst, Holz and Stuenkel

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Electrically evoked chromaffin cell activity. (A) Electrical stimulation of the splanchnic nerve evokes activity in the adrenal medulla. Representative recording of a single phase 10Hz-3.5mA stimulation train (red vertical lines). (B) The local application of TTX to the splanchnic nerve reversibly blocked the evoked action potentials in the adrenal medulla. (C) Evoked templates (solid lines) from two units. The evoked templates are identical to their spontaneous counterparts (dotted lines). (D) The evoked units present distinct latencies that range from 5 to 30 ms after the stimulus (red vertical line). The data are from the same experiments as in panel (C). (E) Distinct networks have different stimulus thresholds. The stimulus intensity was gradually increased from 0 to 6 mA in 0.5 mA steps.