Figure 2.

Effect of MF on LPS-induced activation of STAT3, NF-κB p65, and MAPKs in LPS-challenged mice. Mice were intraperitoneally injected with either different concentrations of MF (50 and 100 mg/kg), Dexa (5 mg/kg), or vehicle (PBS), 1 h before intraperitoneal LPS injection (30 mg/kg). Liver tissue samples were obtained 4 h posttreatment with LPS, to extract protein. (a) Phosphorylated-STAT3, (c) nuclear and cytosolic NF-κB p65, and (e) phosphorylation of JNK and ERK1/2 were analyzed by western blot. (b, d, and f) Densitometric analysis was performed using ImageJ software. The data are presented as mean ± SD. ^###p < 0.001 vs. the control group; ^∗∗∗p < 0.001 vs. the LPS-only-challenged group.