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Effect of MF on phosphorylation of MEK1/2-ERK1/2 and SEK1/MKK4-JNK pathways in LPS-stimulated RAW264.7 macrophages. The cells were pretreated with MF for 1 h and then stimulated with LPS (1 μg/mL) for 10–20 min for analysis of MAPKs. Phosphorylation of MEK1/2-ERK1/2 (a and b) and SEK1/MKK4-JNK (c and d) was determined by western blot. The data are presented as mean ± SD. ###p < 0.001 vs. the control group; ∗∗p < 0.01 and ∗∗∗p < 0.001 vs. the LPS-treated group.
