Figure 1.

Expression of murine IFNα protein and its target genes in syngeneic BLCA cell lines

(A and B) BLCA cell lines were transduced with LV-Ctrl or LV-IFNα vectors at an MOI of 2 and murine IFNα was measured in the cell-free supernatants at 24 h (A) and 72 h (B) after viral transduction. All the three cell lines showed significant expression of IFNα protein in LV-IFNα-transduced cells compared with controls. (C) qPCR analysis of IFNα target genes IRF7, PDL-1, and TRAIL in BLCA cell lines show increased expression following treatment with LV-IFNα virus when compared with control. Not significant (ns), p > 0.05; ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001.